Advanced research on biochemical materials and by Helen Zhang, David Jin, X. J. Zhao

By Helen Zhang, David Jin, X. J. Zhao

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A, Vol. 375-377 (2004), pp. 235-239. B. Lu, Y. Li, F. H. Wang: Corros. , Vol. 48 (2006), pp. 2106-2119. [4] M. K. Tam, S. J. Pang, C. H. Shek: J. Non-Cryst. Solids, Vol. 353 (2007), pp. 3596-3599. [5] D. Zander, B. Heisterkamp, I. Gallino: J. , Vol. 434-435 (2007), pp. 234-236. [6] A. H. Cai, X. Xiong, Y. Liu, W. K. An, G. J. Zhou, Y. Luo, T. L. Li: Mater. Chem. , Vol. 134 (2012), pp. 938-944. [7] A. H. Cai, W. K. An, X. S. Li, Y. Luo, T. L. Li: Adv. Mater. , Vol. 146-147 (2011), pp. 1477-1481.

The electrode was dried at room temperature. 0 V (vs. 5 mM) for five cycles to form the GCE/GS-Thi-Nafion/AuNPs electrode. 3 overnight at 4°C to absorb the antibodies. Finally, the modified immunosensor was incubated in a solution of BSA (10 mg/mL) for about 1 h at room temperature. The immunosensor was stored at 4°C when not in use. CEA could be immobilized on the anti-CEA-modified GCE surface because of the strong affinity between CEA and anti-CEA. The attachment of each component was monitored via the electrochemistry.

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